Redox, Development and Adaptation to Constraints (ReDAC)

Responsible Jean-Philippe REICHHELD (DR2 CNRS)

Understanding the mechanism of plant adaptation to environmental constraints is key in the context of climate change which affects plant performance, vigour, and/or yield. Cellular redox modifications caused by compounds such as Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) are important factor of these mechanisms. The transmission of oxidative signals occurs notably through posttranslational modifications of thiols residues of cysteines of numerous proteins. These modifications of different nature (disulfide bonds, glutathionylation, nitrosylation, sulhydration, sulfenylation,…) act on protein structure and activities, and might influence many associated functions. Thiol reduction systems like glutathione and the thiol reductases glutaredoxins (GRX) and thioredoxins (TRX) are major actors regulating thiol redox status.

Our research group study for many years these compounds. These researches are addressed mostly in Arabidopsis thaliana using biochemical, genetic and genomic approaches. Our works have contributed to the identification and classification of these proteins. We have developed genetic and proteomic tools to characterize the biological functions and the targets of these proteins. Particularly, we have demonstrated the implication of thiol reductases in different plant development pathways, hormonal signaling, carbon metabolism and responses to environmental constraints.

 

Some recent results and research perspectives:

- identification thiol reduction systems in the nucleus (Marchal et al, 2014 ; Knuesting et al, 2015) ; Delorme-Hinoux et al, 2016). These works initiated in the context of the ANR-Nucleoredox project open new perspectives to find redox regulated pathways in the nucleus, uncluding gene expression and chromatin modeling upon heat stress (cf ANR-REPHARE proposal).

- demonstration, in collaboration with the group of Julio Saez-Vasquez, of a redox regulation of DICER-like RNAseIII and its possible role in siRNA metabolism (Charbonnel, Niazi et al, 2017).

- evidence for the protecting functions of NRX1 and GRXS17 in oxidative and thermotolerance (Kneeshaw et al, 2017 ; Martins et al, 2019).

- role of the mitochondrial TRX system as a master regulator of the TCA cycle, the redox regulation of  carbon metabolism enzymes and in drough stress tolerance (Daloso et al, 2015; Huang et al, 2018; Niazi et al, 2019; da Fonseca-Pereira et al., 2019).

- key role of glutathione and thioredoxin system in root development and auxins signaling  (Bashandy et al, 2010; Trujillo-Hernandez et al, 2019).

    equipe reichheld

    Team :

BARIAT Laëtitia - Assistant Ingénieur CNRS

BELIN Christophe - Maître de conférences UPVD

DARD Avilien - Doctorant

REICHHELD Jean-Philippe - Directeur de recherche CNRS

RIONDET Christophe - Maître de conférences UPVD

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